4EWP

Crystal structure of FabH from Micrococcus luteus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	298
Detector technology	CCD
Collection date	2010-03-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9774
Spacegroup name	P 1
Unit cell lengths	80.140, 85.026, 96.916
Unit cell angles	69.82, 71.37, 85.66

Refinement procedure

Resolution	49.797 - 2.198
R-factor	0.1709
Rwork	0.169
R-free	0.20980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.071
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: dev_718))

Data quality characteristics

	Overall
Low resolution limit [Å]	49.800
High resolution limit [Å]	2.198
Completeness [%]	96.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	298	0.1 M Bis-Tris propane pH 9.0, 10% PEG 200, 18% PEG 8,000, VAPOR DIFFUSION, SITTING DROP, temperature 298K