4EWF
The crystal structure of beta-lactamase from Sphaerobacter thermophilus DSM 20745
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97924 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 137.500, 215.551, 83.816 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.328 - 2.000 |
R-factor | 0.1824 |
Rwork | 0.181 |
R-free | 0.20950 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.086 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MLPHARE |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 84422 | |
<I/σ(I)> | 10.5 | 2.84 |
Completeness [%] | 98.9 | 98.7 |
Redundancy | 6.3 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.1M Bis-Tris Propane, NaOH pH 7.0 1.5 M Ammonium Sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |