4EWC

Crystal Structure of the Infectious Salmon Anemia Virus Nucleoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-01
Detector	NOIR-1
Wavelength(s)	0.97895
Spacegroup name	P 41 21 2
Unit cell lengths	112.983, 112.983, 103.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.978 - 2.700
R-factor	0.2679
Rwork	0.268
R-free	0.27060
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.133
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.700	2.700
Rmerge	0.120	0.458
Number of reflections	18972
<I/σ(I)>	9.8	2.9
Completeness [%]	99.9	99.8
Redundancy	5.4	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4	283	20% PEG6000, 0.8M Lithium Chloride, 0.1M Sodium Citrate, pH 4.0, VAPOR DIFFUSION, temperature 283K