4EW5

C-terminal domain of inner membrane protein CigR from Salmonella enterica.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	49.870, 54.811, 73.992
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.400 - 1.870
R-factor	0.1783
Rwork	0.176
R-free	0.23010
Structure solution method	SAD
RMSD bond length	0.019
RMSD bond angle	1.830
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.400	50.000	1.910
High resolution limit [Å]	1.870	5.100	1.880
Rmerge	0.107	0.072	0.811
Number of reflections	17270
<I/σ(I)>	8.9		2.2
Completeness [%]	99.9	99.1	100
Redundancy	6.8	6.3	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289	0.1M Hepes buffer, 2% PEG-400, 2M Ammonium sulphate. Full length protein was crystallized in presence of V8 protease., pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K