4EVZ
Structure of HisF-LUCA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-11-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.074, 90.736, 57.369 |
| Unit cell angles | 90.00, 100.25, 90.00 |
Refinement procedure
| Resolution | 47.934 - 1.462 |
| R-factor | 0.1526 |
| Rwork | 0.151 |
| R-free | 0.17610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1thf |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.294 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CCP4 (suite) |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 56.454 |
| High resolution limit [Å] | 1.460 |
| Rmerge | 0.050 |
| Number of reflections | 69542 |
| <I/σ(I)> | 19.12 |
| Completeness [%] | 92.0 |
| Redundancy | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 20% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
