4EVZ
Structure of HisF-LUCA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-11-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.074, 90.736, 57.369 |
Unit cell angles | 90.00, 100.25, 90.00 |
Refinement procedure
Resolution | 47.934 - 1.462 |
R-factor | 0.1526 |
Rwork | 0.151 |
R-free | 0.17610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1thf |
RMSD bond length | 0.010 |
RMSD bond angle | 1.294 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | CCP4 (suite) |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 56.454 |
High resolution limit [Å] | 1.460 |
Rmerge | 0.050 |
Number of reflections | 69542 |
<I/σ(I)> | 19.12 |
Completeness [%] | 92.0 |
Redundancy | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 20% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 291K |