4EVU
Crystal structure of C-terminal domain of putative periplasmic protein ydgH from S. enterica
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97935 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.079, 52.521, 39.139 |
Unit cell angles | 90.00, 109.38, 90.00 |
Refinement procedure
Resolution | 36.920 - 1.450 |
R-factor | 0.13437 |
Rwork | 0.133 |
R-free | 0.17157 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.450 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MLPHARE |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.480 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.079 | 0.576 |
Number of reflections | 26374 | |
<I/σ(I)> | 25.2 | 2.2 |
Completeness [%] | 99.8 | 100 |
Redundancy | 3.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 295 | 0.1 M Na Citrate , 0.2 M K/Na tartrate, 1.9 M (NH4)2SO4, 5% Glycerol, 1/210 trypsin, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 295K |