4EVF
Crystal structure of apo alpha-1 giardin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-17 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.488 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.267, 58.747, 99.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.826 - 1.900 |
| R-factor | 0.2026 |
| Rwork | 0.199 |
| R-free | 0.26240 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.972 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 47848 | |
| Completeness [%] | 1.0 | 97.2 |
| Redundancy | 5.1 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | 0.1M MgCl2, 30% PEG 2000, 20mM KH2PO4, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
| 2 |
