4EVC
Crystal Structure HP-NAP from strain YS39 cadmium loaded (Cocrystallization 50mM)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU ULTRAX 18 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-05-28 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 186.711, 186.711, 186.711 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.2218 |
| Rwork | 0.219 |
| R-free | 0.25040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ta8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.015 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.300 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.099 | 0.288 |
| Number of reflections | 11426 | |
| <I/σ(I)> | 10.1 | 5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 50mM cadmium sulfate, 1.0M sodium acetate, 0.1M HEPES-NaOH, 0.1M L-Arginine, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
