4EVB
Crystal Structure HP-NAP from strain YS39 zinc soaked (20mM)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2009-11-20 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.2892 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 185.529, 185.529, 185.529 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.2335 |
| Rwork | 0.227 |
| R-free | 0.29110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ta8 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.471 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.046 | 0.371 |
| Number of reflections | 9990 | |
| <I/σ(I)> | 63.6 | 9.4 |
| Completeness [%] | 99.2 | 100 |
| Redundancy | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 293 | 0.1M L-Arginine, 20% ethylene glycol, 20mM zinc sulfate, pH 8.8, vapor diffusion, hanging drop, temperature 293K |
