4EV2
Crystal structure of copper amine oxidase-1 from Hansenula polymorpha in complex with ethylamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 104.408, 232.834, 105.119 |
| Unit cell angles | 90.00, 96.66, 90.00 |
Refinement procedure
| Resolution | 48.970 - 2.180 |
| R-factor | 0.1528 |
| Rwork | 0.150 |
| R-free | 0.20129 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4ev5 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.594 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | DF |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.220 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Rmerge | 0.108 | 0.457 |
| Number of reflections | 257277 | |
| <I/σ(I)> | 10 | 1.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 8% PEG8000, 0.22 M potassium phosphate, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
