4EV1
Anabaena Tic22 (protein transport)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.933 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 67.168, 111.190, 44.152 |
| Unit cell angles | 90.00, 125.36, 90.00 |
Refinement procedure
| Resolution | 36.010 - 1.950 |
| R-factor | 0.1997 |
| Rwork | 0.197 |
| R-free | 0.24897 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.514 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.010 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 19161 | |
| <I/σ(I)> | 18.9 | 2.4 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 4.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 292 | 1 M sodium citrate, 0.1 M CHES , pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 292.0K |
| 2 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 292 | 1 M sodium citrate, 0.15mM MgCl2 0.1 M CHES, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 292.0K |
