4ETZ
Crystal Structure of PelD 158-CT from Pseudomonas aeruginosa PAO1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Detector technology | IMAGE PLATE |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.320, 41.310, 110.850 |
Unit cell angles | 90.00, 95.45, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.050 |
R-factor | 0.2328 |
Rwork | 0.231 |
R-free | 0.27740 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.510 |
Data scaling software | XSCALE |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 2.150 | ||
High resolution limit [Å] | 2.050 | 50.000 | 2.050 |
Rmerge | 0.060 | 0.897 | |
Number of reflections | 40304 | 5315 | |
<I/σ(I)> | 15.68 | 2.31 | |
Completeness [%] | 99.7 | 99.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 8.5 | 298 | 100 mM Tris, pH 8.5, 200 mM Li2SO4, and 1.26 M (NH4)2SO4, hanging drop, temperature 298K |