4ETU
Crystal structure of rabbit ryanodine receptor 1 mutant R2939S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-08 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 32.341, 56.463, 116.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 116.420 - 2.190 |
| R-factor | 0.2137 |
| Rwork | 0.211 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ert |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.299 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 116.420 | 50.000 | 2.280 |
| High resolution limit [Å] | 2.190 | 4.740 | 2.200 |
| Rmerge | 0.062 | 0.045 | 0.326 |
| Number of reflections | 11073 | ||
| <I/σ(I)> | 12.6 | ||
| Completeness [%] | 96.2 | 99.5 | 77.2 |
| Redundancy | 3.5 | 5 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 15% PEG3350, 25% Glycerol, 0.1M HEPES, pH 7.0, vapor diffusion, hanging drop, temperature 295K |
