4ETS
Crystal structure of Campylobacter jejuni ferric uptake regulator
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2010-09-09 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.07820 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.954, 85.284, 128.435 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.760 - 2.100 |
R-factor | 0.23731 |
Rwork | 0.236 |
R-free | 0.26579 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.023 |
RMSD bond angle | 1.897 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.100 |
Number of reflections | 23938 |
Completeness [%] | 100.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6 | 298 | 200mM MnSO4, 10-20% PEG3350, pH 6.0, EVAPORATION, temperature 298K |