4ETS

Crystal structure of Campylobacter jejuni ferric uptake regulator

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	200
Detector technology	CCD
Collection date	2010-09-09
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.07820
Spacegroup name	P 21 21 21
Unit cell lengths	35.954, 85.284, 128.435
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.760 - 2.100
R-factor	0.23731
Rwork	0.236
R-free	0.26579
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.023
RMSD bond angle	1.897
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.100
Number of reflections	23938
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	298	200mM MnSO4, 10-20% PEG3350, pH 6.0, EVAPORATION, temperature 298K