4ETM
Crystal structure of YfkJ from Bacillus subtilis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BSRF BEAMLINE 3W1A |
Synchrotron site | BSRF |
Beamline | 3W1A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-15 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 70.152, 52.922, 86.609 |
Unit cell angles | 90.00, 102.84, 90.00 |
Refinement procedure
Resolution | 21.368 - 1.600 |
R-factor | 0.226 |
Rwork | 0.225 |
R-free | 0.24850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5pnt |
RMSD bond length | 0.007 |
RMSD bond angle | 1.110 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 40047 | |
Completeness [%] | 98.2 | 98.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.05 | 291 | 1.344M dipotassium phosphate, 0.056M monosodium phosphate, pH 8.05, VAPOR DIFFUSION, HANGING DROP, temperature 291K |