4ETL
Crystallographic structure of phenylalanine hydroxylase from Chromobacterium violaceum F258A mutation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-03-05 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.033 |
Spacegroup name | P 1 |
Unit cell lengths | 36.780, 38.553, 48.044 |
Unit cell angles | 76.47, 73.00, 85.36 |
Refinement procedure
Resolution | 22.680 - 1.490 |
R-factor | 0.16727 |
Rwork | 0.165 |
R-free | 0.21421 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ltu |
RMSD bond length | 0.007 |
RMSD bond angle | 1.248 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.520 |
High resolution limit [Å] | 1.490 | 1.490 |
Rmerge | 0.059 | |
Number of reflections | 36922 | |
<I/σ(I)> | 20.1 | |
Completeness [%] | 92.0 | 59.7 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1M Na-HEPES, 0.001M Magnesium chloride hexahydrate, 0.005M Nickel (II) chloride hexahydrate, 15% w/v PEG 3,350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |