4ETI

Crystal Structure of YwlE from Bacillus subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-02-01
Detector	MAR CCD 165 mm
Wavelength(s)	1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	84.736, 38.764, 40.463
Unit cell angles	90.00, 91.12, 90.00

Refinement procedure

Resolution	18.917 - 1.800
R-factor	0.1808
Rwork	0.178
R-free	0.20130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zgg
RMSD bond length	0.003
RMSD bond angle	0.769
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.000	1.900
High resolution limit [Å]	1.800	1.800
Number of reflections	11539
Completeness [%]	93.4	93.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	0.2M HCOONa, 20% PEG 3350, 0.1M spermidine, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K