4ETC
Lysozyme, room temperature, 24 kGy dose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2012-03-25 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9997 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.290, 79.290, 38.120 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.400 - 1.906 |
R-factor | 0.1678 |
Rwork | 0.166 |
R-free | 0.20010 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.007 |
RMSD bond angle | 1.048 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.400 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 9297 | |
Completeness [%] | 92.6 | 95.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | grown in Linbro plates in hanging or sitting drop geometry. Drops of equal volume of protein (20 mg/ml) and reservoir solution (1 M NaAc pH 3.0, 9-10 % NaCl, 6 % PEG 6000) were mixed. For the measurements, the crystals were equilibrated in 1 M NaAc pH 3.4, 10 % NaCl, VAPOR DIFFUSION, temperature 293K |