4ESY
Crystal Structure of the CBS Domain of CBS Domain Containing Membrane Protein from Sphaerobacter thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-02-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97931 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.090, 74.118, 50.981 |
Unit cell angles | 90.00, 99.13, 90.00 |
Refinement procedure
Resolution | 38.845 - 2.011 |
R-factor | 0.184 |
Rwork | 0.180 |
R-free | 0.22600 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.419 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((phenix.refine: 1.7.3_920)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 24825 | |
<I/σ(I)> | 10.4 | 2.1 |
Completeness [%] | 99.0 | 89.6 |
Redundancy | 5.3 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1 M MES pH 7.5, 10 %(w/v) PEG 8000, 8 %(v/v) Ethylene Glycol, VAPOR DIFFUSION, SITTING DROP, temperature 298K |