4ESV

A New Twist on the Translocation Mechanism of Helicases from the Structure of DnaB with its Substrates

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	1.11
Spacegroup name	P 2 2 21
Unit cell lengths	149.124, 180.321, 279.128
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.920 - 3.200
R-factor	0.245
Rwork	0.242
R-free	0.28900
Structure solution method	SIRAS
RMSD bond length	0.011
RMSD bond angle	1.506
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.420
High resolution limit [Å]	3.200	7.100	3.200
Rmerge	0.115	0.044
Number of reflections	124032
<I/σ(I)>	6.5
Completeness [%]	88.3	89.3	87.6
Redundancy	3	3.1	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	290	4-7% PEG3350, 0.2 M calcium chloride, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K
1	VAPOR DIFFUSION, SITTING DROP	6.5	290	4-7% PEG3350, 0.2 M calcium chloride, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K
1	VAPOR DIFFUSION, SITTING DROP	6.5	290	4-7% PEG3350, 0.2 M calcium chloride, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K