4ESU
Crystal structure of rabbit ryanodine receptor 1 mutant S2776M
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-11-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 32.154, 56.193, 113.775 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.890 - 1.590 |
R-factor | 0.198 |
Rwork | 0.196 |
R-free | 0.22930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ert |
RMSD bond length | 0.013 |
RMSD bond angle | 1.097 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 56.890 | 1.640 | |
High resolution limit [Å] | 1.590 | 7.130 | 1.590 |
Rmerge | 0.065 | 0.027 | 0.590 |
Number of reflections | 27184 | 346 | 1836 |
<I/σ(I)> | 12.47 | 33.84 | 2.09 |
Completeness [%] | 95.6 | 87.6 | 89.2 |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 25% PEG3350, 25% Glycerol, 0.1M HEPES, pH 7.0, vapor diffusion, hanging drop, temperature 295K |