4ESK
Crystal structure of a strand-swapped dimer of Mouse Leukocyte-associated immunoglobulin-like receptor 1 (NYSGRC-006047)IG-like domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0750 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.141, 73.121, 90.609 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.510 - 1.762 |
R-factor | 0.1853 |
Rwork | 0.184 |
R-free | 0.21610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ety |
RMSD bond length | 0.009 |
RMSD bond angle | 1.354 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.510 | 1.790 |
High resolution limit [Å] | 1.760 | 1.760 |
Number of reflections | 40723 | |
<I/σ(I)> | 59.3 | 6.7 |
Completeness [%] | 97.5 | 85.8 |
Redundancy | 14.5 | 13.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Sitting Drop Vapor Diffusion | 6 | 298 | Protein (8.5mg/ml in 20 mM Trizma Base pH 8.2, 100 mM NaCl; Reservoir (1.0 M K/Na tartrate MES pH 6.0 - Wizard I & II #98); Cryoprotection (30% glycerol in Reservior solution), Sitting Drop Vapor Diffusion, temperature 298K |