4ES2
Double-stranded Endonuclease Activity in B. halodurans Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated Cas2 Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-29 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9770 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 66.078, 33.509, 39.163 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.050 - 1.299 |
R-factor | 0.1707 |
Rwork | 0.168 |
R-free | 0.19190 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.407 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX (1.7_650) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.050 | 1.320 |
High resolution limit [Å] | 1.299 | 1.299 |
Number of reflections | 21058 | |
Completeness [%] | 95.2 | 83.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294 | 50 mM MES, pH 6.0, 2% w/v MPD, 10 mM magnesium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 294K |