4ES1
Double-stranded Endonuclease Activity in B. halodurans Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated Cas2 Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-29 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9770 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 65.887, 33.440, 39.093 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.100 |
R-factor | 0.15807 |
Rwork | 0.158 |
R-free | 0.16435 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.027 |
RMSD bond angle | 2.266 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 1.120 |
High resolution limit [Å] | 1.100 | 1.100 |
Number of reflections | 34580 | |
Completeness [%] | 96.6 | 86.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294 | 50 mM MES, pH 6.0, 2% w/v PEG2000 MME, 10 mM magnesium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 294K |