4ERY
X-ray structure of WDR5-MLL3 Win motif peptide binary complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Detector technology | CCD |
Collection date | 2009-10-01 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.917 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.530, 68.250, 87.880 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.940 - 1.300 |
R-factor | 0.1878 |
Rwork | 0.187 |
R-free | 0.20910 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.178 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.240 |
High resolution limit [Å] | 1.220 | 3.310 | 1.220 |
Rmerge | 0.081 | 0.063 | 0.634 |
Number of reflections | 83725 | ||
<I/σ(I)> | 11.9 | ||
Completeness [%] | 99.6 | 96.2 | 96.9 |
Redundancy | 12.3 | 12.8 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 7.5 | 294 | PEG3350, Ammonium Sulfate, HEPES, pH 7.5, hanging drop, temperature 294K |