4ERV
Crystal structure of human ryanodine receptor 3 (2597-2800)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-29 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.570, 58.540, 47.460 |
| Unit cell angles | 90.00, 97.21, 90.00 |
Refinement procedure
| Resolution | 35.350 - 1.750 |
| R-factor | 0.1914 |
| Rwork | 0.190 |
| R-free | 0.22010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.214 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 | |
| High resolution limit [Å] | 1.750 | 7.830 | 1.750 |
| Rmerge | 0.035 | 0.027 | 0.452 |
| Number of reflections | 25284 | 301 | 1871 |
| <I/σ(I)> | 20.3 | 47.13 | 3.15 |
| Completeness [%] | 98.1 | 97.7 | 99.2 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 295 | 1.4M Ammonium sulfate, 0.1M Bicine, pH 9.5, vapor diffusion, hanging drop, temperature 295K |
