4ERQ
X-ray structure of WDR5-MLL2 Win motif peptide binary complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Detector technology | CCD |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.917 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 139.780, 80.790, 88.120 |
Unit cell angles | 90.00, 90.20, 90.00 |
Refinement procedure
Resolution | 29.775 - 1.906 |
R-factor | 0.1671 |
Rwork | 0.165 |
R-free | 0.19970 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.137 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 1.900 |
Number of reflections | 76235 |
<I/σ(I)> | 9 |
Completeness [%] | 1.0 |
Redundancy | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 7.3 | 294 | PEG3350, Ammonium Sulfate, HEPES, pH 7.3, hanging drop, temperature 294K |