4EQQ

Structure of Ltp, a superinfection exclusion protein from the Streptococcus thermophilus temperate phage TP-J34

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-02-03
Detector	PSI PILATUS 6M
Wavelength(s)	0.95373
Spacegroup name	P 41 21 2
Unit cell lengths	82.770, 82.770, 100.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.380 - 2.050
R-factor	0.1636
Rwork	0.163
R-free	0.17870
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	0.940
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.11.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.100
High resolution limit [Å]	2.050	2.050
Rmerge	0.073	0.570
Number of reflections	22479
<I/σ(I)>	26.7	5.2
Completeness [%]	100.0	100
Redundancy	14.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	The perecipitant liquor contained Ammonium sulfate 1.50M in tri-Sodium citrate 00.15M, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K