4EQM
Structural analysis of Staphylococcus aureus serine/threonine kinase PknB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-10-10 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 221.510, 127.550, 70.280 |
| Unit cell angles | 90.00, 89.96, 90.00 |
Refinement procedure
| Resolution | 43.500 - 3.000 |
| R-factor | 0.2165 |
| Rwork | 0.215 |
| R-free | 0.24610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.918 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.500 | 3.080 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 38868 | |
| Completeness [%] | 99.1 | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 0.06M magnesium chloride, 0.08M MES, 1.3M sodium citrate, 2% benzamidine hydrochloride, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 277.0K |
