4EQK
Crystal structure of the cap-binding domain of polymerase basic protein 2 from influenza virus A/Hong Kong/1/68 (h3n2) with bound m7GTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-28 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9795 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 55.379, 70.151, 93.941 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.260 - 1.950 |
R-factor | 0.18656 |
Rwork | 0.183 |
R-free | 0.24627 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vqz |
RMSD bond length | 0.022 |
RMSD bond angle | 2.258 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 1.950 |
Number of reflections | 12525 |
Completeness [%] | 91.7 |
Redundancy | 11.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.2M lithium nitrate, 0.1M Tris-HCl pH 8.0, 18% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |