4EQF
Trip8b-1a#206-567 interacting with the carboxy-terminal seven residues of HCN2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 72.320, 72.320, 146.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.808 - 3.000 |
| R-factor | 0.2293 |
| Rwork | 0.227 |
| R-free | 0.28070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fch |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.135 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX (1.7.1_743) |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.910 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.092 | 0.990 |
| Number of reflections | 8364 | |
| <I/σ(I)> | 15.1 | 2.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13 | 13.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 91mM MES, 91mM triSodium citrate, 3.63M NaCL, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
