4EQ6
The crystal structure of Psy3-Csm2 complex from budding yeast
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 135.333, 51.162, 77.049 |
| Unit cell angles | 90.00, 103.02, 90.00 |
Refinement procedure
| Resolution | 29.855 - 1.800 |
| R-factor | 0.2014 |
| Rwork | 0.201 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.137 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.790 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.043 | 0.542 |
| Number of reflections | 43595 | |
| <I/σ(I)> | 38.9 | 2.5 |
| Completeness [%] | 94.0 | 91.9 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 100mM Tris-HCl, pH 8.0, 10% ethanol, 10% 2-methyl-2,4-pentadiol (MPD), VAPOR DIFFUSION, SITTING DROP, temperature 293K |
