4EQ3
Crystal Structure Analysis of Selenomethionine (Se-Met) Substituted Chicken Interferon Gamma Receptor Alpha Chain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97923, 0.9789, 0.9791, 0.9000 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 63.608, 63.608, 215.750 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.539 - 2.001 |
| R-factor | 0.2347 |
| Rwork | 0.233 |
| R-free | 0.26020 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.288 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.067 | 0.302 |
| Number of reflections | 17153 | |
| <I/σ(I)> | 59.328 | 18.095 |
| Completeness [%] | 97.0 | 97.1 |
| Redundancy | 32.6 | 35.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 20% (v/v) polyethylene glycol 5000 monomethyl ether, 0.1M HEPES, 0.5M sodium carbonate , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
