4EPZ
Crystal structure of a transcription anti-terminator antagonist UpxZ (BACUNI_04315) from Bacteroides uniformis ATCC 8492 at 1.68 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-04-04 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 42.253, 81.110, 105.702 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.864 - 1.680 |
R-factor | 0.1695 |
Rwork | 0.168 |
R-free | 0.19630 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 0.910 |
Data reduction software | XDS |
Data scaling software | XSCALE (December 29, 2011) |
Phasing software | SHELX |
Refinement software | BUSTER-TNT (2.10.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 37.864 | 37.860 | 1.740 |
High resolution limit [Å] | 1.680 | 3.610 | 1.680 |
Rmerge | 0.065 | 0.040 | 0.678 |
Number of reflections | 20732 | 2211 | 2038 |
<I/σ(I)> | 11.8 | 25.5 | 2.35 |
Completeness [%] | 97.9 | 97.1 | 98.1 |
Redundancy | 4.29 | 4.53 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2M calcium acetate, 20.0% polyethylene glycol 3350, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |