4EPU

Ang1 fibrinogen-related domain (FReD)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-07-31
Detector	MAR scanner 300 mm plate
Wavelength(s)	1.0781
Spacegroup name	P 64
Unit cell lengths	80.903, 80.903, 186.087
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.443 - 2.098
R-factor	0.1835
Rwork	0.183
R-free	0.20080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1z3s
RMSD bond length	0.007
RMSD bond angle	1.075
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.7_650)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.098
Number of reflections	39116

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1M PIPES pH 6.5, 0.2M NaCl, 2M ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 291K