4EPC
Crystal structure of Autolysin repeat domains from Staphylococcus epidermidis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-28 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.934 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 95.360, 95.360, 233.670 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.650 - 2.900 |
R-factor | 0.2656 |
Rwork | 0.263 |
R-free | 0.29840 |
Structure solution method | MIR |
RMSD bond length | 0.010 |
RMSD bond angle | 1.230 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SOLVE |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.650 | 2.980 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 14506 | |
Completeness [%] | 98.9 | 99.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 10.5 | 293 | 1.2 M NaH2PO4, 0.8 M K2HPO4, 0.2 M LiSO4, 0.1 M CAPS, pH 10.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |