4EOY
Plasmodium falciparum Atg8 in complex with Plasmodium falciparum Atg3 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-02-20 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 33.300, 111.080, 57.090 |
Unit cell angles | 90.00, 92.58, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.220 |
R-factor | 0.20839 |
Rwork | 0.205 |
R-free | 0.27965 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 20 models (15 PDB 5 predicted) as ensemble using phenix.automr |
RMSD bond length | 0.008 |
RMSD bond angle | 1.197 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX (Phaser) |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.060 | 2.240 |
High resolution limit [Å] | 2.220 | 2.180 |
Rmerge | 0.215 | 0.918 |
Number of reflections | 17965 | |
<I/σ(I)> | 7.28 | 1.6 |
Completeness [%] | 83.4 | 45 |
Redundancy | 2.07 | 2.18 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 0.1 M MES pH 6.5, 0.1 M Ca(OAc)2, 20% w/v PEG 8000, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 0.1 M MES pH 6.5, 0.1 M Ca(OAc)2, 20% w/v PEG 8000, VAPOR DIFFUSION, SITTING DROP, temperature 295K |