4EOG

Crystal structure of Csx1 of Pyrococcus furiosus

Experimental procedure

Experimental method	MAD
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2010-12-25
Detector	RIGAKU
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	51.820, 179.530, 110.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.080 - 2.300
R-factor	0.227
Rwork	0.227
R-free	0.25000
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	CNS (1.3)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.300
Number of reflections	22968
Completeness [%]	99.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	298	0.1 M MES, 0.01 M Zinc sulfate, 6 % PEG MME 550, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
2	VAPOR DIFFUSION, HANGING DROP	6	298	0.1 M MES, 0.01 M Zinc sulfate, 6 % PEG MME 550, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K