4EOE
Crystal structure of H74A synechocystis sp. pcya
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 70.571, 95.194, 42.726 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.000 - 1.200 |
| R-factor | 0.14993 |
| Rwork | 0.149 |
| R-free | 0.17084 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.595 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 1.210 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.050 | 0.305 |
| Number of reflections | 88225 | |
| <I/σ(I)> | 14 | 2.4 |
| Completeness [%] | 93.1 | 67 |
| Redundancy | 3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 1-1.25 M sodium citrate, 0.1-0.4 M NaCl in 0.1 M Tris HCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
