4EOD
Crystal structure of H74E synechocystis sp. pcya sp. PCYA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.09717 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 70.638, 95.530, 42.767 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.570 - 1.300 |
| R-factor | 0.16632 |
| Rwork | 0.165 |
| R-free | 0.18828 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.597 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.000 | 1.330 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.046 | 0.434 |
| Number of reflections | 70757 | |
| <I/σ(I)> | 15.73 | 3.25 |
| Completeness [%] | 98.2 | 97.2 |
| Redundancy | 3.55 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 298 | 1-1.25 M sodium citrate, 0.1-0.4 M NaCl in 0.1 M Tris HCl, pH 7, VAPOR DIFFUSION, temperature 298K |
