4EOB
Structure of the type VI peptidoglycan amidase effector Tse1 from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.662, 107.578, 84.396 |
| Unit cell angles | 90.00, 94.23, 90.00 |
Refinement procedure
| Resolution | 45.320 - 2.611 |
| Rwork | 0.186 |
| R-free | 0.24440 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.130 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (Phaser) |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.320 | 2.660 |
| High resolution limit [Å] | 2.610 | 2.610 |
| Rmerge | 0.234 | |
| Number of reflections | 20654 | |
| <I/σ(I)> | 7.81 | |
| Completeness [%] | 98.4 | 94.3 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 297 | 0.1M HEPES pH 7.5, 100mM Sodium thiocyanate, 18% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
