4ENZ
Structure of human ceruloplasmin at 2.6 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-05-26 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.98 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 210.775, 210.775, 84.502 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 14.900 - 2.600 |
R-factor | 0.2007 |
Rwork | 0.199 |
R-free | 0.23436 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2j5w |
RMSD bond length | 0.017 |
RMSD bond angle | 1.673 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.700 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 59625 | |
Completeness [%] | 95.2 | 92.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 280 | 40% MPD, 100 mM sodium chloride, 10 mM calcium chloride, 0.1 M sodium acetate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 280K |