4ENZ

Structure of human ceruloplasmin at 2.6 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2006-05-26
Detector	MAR CCD 165 mm
Wavelength(s)	0.98
Spacegroup name	P 32 2 1
Unit cell lengths	210.775, 210.775, 84.502
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	14.900 - 2.600
R-factor	0.2007
Rwork	0.199
R-free	0.23436
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2j5w
RMSD bond length	0.017
RMSD bond angle	1.673
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.700
High resolution limit [Å]	2.600	2.600
Number of reflections	59625
Completeness [%]	95.2	92.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	280	40% MPD, 100 mM sodium chloride, 10 mM calcium chloride, 0.1 M sodium acetate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 280K