4ENN
Crystal structure of S. pombe Atl1 in complex with damaged DNA containing O6-carboxymethylguanine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-08-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1158 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 85.154, 85.154, 150.715 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.269 - 2.845 |
R-factor | 0.2238 |
Rwork | 0.217 |
R-free | 0.27830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gva |
RMSD bond length | 0.010 |
RMSD bond angle | 1.802 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.950 |
High resolution limit [Å] | 2.845 | 2.845 |
Number of reflections | 13069 | |
Completeness [%] | 95.6 | 77 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 288 | 20% mPEG 2000, 5% sodium formate, 25% sodium chloride, 0.2M imidazole-malate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 288K |