4EMK

Crystal structure of SpLsm5/6/7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-08-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 41 21 2
Unit cell lengths	69.430, 69.430, 172.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.053 - 2.300
R-factor	0.2323
Rwork	0.231
R-free	0.25390
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.247
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	86.160	2.420
High resolution limit [Å]	2.300	2.300
Number of reflections	19618
Completeness [%]	100.0	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	288	0.1M MES, pH 6.5, 0.1M MgCl2, 32% polyethylene glycol (PEG) 400, VAPOR DIFFUSION, HANGING DROP, temperature 288K