4EMH
Crystal structure of SpLsm4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 185.121, 124.470, 131.567 |
| Unit cell angles | 90.00, 135.07, 90.00 |
Refinement procedure
| Resolution | 92.559 - 2.200 |
| R-factor | 0.2376 |
| Rwork | 0.237 |
| R-free | 0.25270 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.308 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.910 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 105873 | |
| Completeness [%] | 99.3 | 91.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | 0.1M MES, pH6.5, 0.1M NaCl, 12% polyethylene glycol (PEG) 4000, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
