Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-03-15 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.8423 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.505, 94.590, 120.502 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 16.939 - 2.200 |
| R-factor | 0.1974 |
| Rwork | 0.195 |
| R-free | 0.24720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3KU8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.082 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 16.940 |
| High resolution limit [Å] | 2.131 |
| Rmerge | 0.156 |
| Number of reflections | 32823 |
| <I/σ(I)> | 15 |
| Completeness [%] | 100.0 |
| Redundancy | 44.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10 | 293 | 1.2M NAH2PO4, 0.8M K2HPO4, 100MM CAPS, pH 10.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
