4ELR

Ferric binding protein with iron and carbonate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-01
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	P 31 2 1
Unit cell lengths	63.602, 63.602, 266.701
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.465 - 2.502
R-factor	0.1712
Rwork	0.169
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.050
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_596))

Data quality characteristics

	Overall
Low resolution limit [Å]	42.465
High resolution limit [Å]	2.500
Number of reflections	22650
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.2M MgCl2, 0.1M Hepes pH 7.5, 26% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K