4EL8
The unliganded structure of C.bescii CelA GH48 module
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-10 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.300, 57.666, 106.060 |
| Unit cell angles | 90.00, 102.30, 90.00 |
Refinement procedure
| Resolution | 51.810 - 2.450 |
| R-factor | 0.1986 |
| Rwork | 0.195 |
| R-free | 0.25812 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g9g |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.243 |
| Data reduction software | PROTEUM PLUS (PLUS) |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Phasing software | MrBUMP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.810 | 2.540 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Number of reflections | 25544 | |
| <I/σ(I)> | 13.57 | 2.17 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.72 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 294 | 0.1 M Tri-sodium citrate pH 5.8 and 20% (w/v) PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
