4EKT
Final Thaumatin Structure for Radiation Damage Experiment at 180 K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 2010-06-22 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.917 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 57.900, 57.900, 150.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.660 - 1.750 |
| R-factor | 0.1752 |
| Rwork | 0.174 |
| R-free | 0.20160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.070 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | BUSTER (2.8.0) |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 3.770 | 1.750 |
| Rmerge | 0.088 | 0.066 | 0.447 |
| Number of reflections | 26070 | ||
| <I/σ(I)> | 7.4 | ||
| Completeness [%] | 97.5 | 94 | 95.3 |
| Redundancy | 5.7 | 5.8 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | sodium potassium tartrate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
