4EKJ
Crystal structure of a monomeric beta-xylosidase from Caulobacter crescentus CB15
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE W01B-MX2 |
Synchrotron site | LNLS |
Beamline | W01B-MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-09-11 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.46 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 71.185, 71.185, 226.703 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.700 - 2.500 |
R-factor | 0.1913 |
Rwork | 0.188 |
R-free | 0.24816 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1w91 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.277 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.700 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 20621 | |
Completeness [%] | 97.5 | 97.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | ammonium sulphate cobalt chloride, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |